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sfg retroviral vector backbone  (TaKaRa)


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    TaKaRa sfg retroviral vector backbone
    Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of <t>retroviral</t> vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.
    Sfg Retroviral Vector Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sfg retroviral vector backbone/product/TaKaRa
    Average 99 stars, based on 9510 article reviews
    sfg retroviral vector backbone - by Bioz Stars, 2026-02
    99/100 stars

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    1) Product Images from "Transgenic CD8αβ co-receptor rescues endogenous TCR function in TCR-transgenic virus-specific T cells"

    Article Title: Transgenic CD8αβ co-receptor rescues endogenous TCR function in TCR-transgenic virus-specific T cells

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2020-001487

    Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of retroviral vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.
    Figure Legend Snippet: Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of retroviral vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.

    Techniques Used: Transgenic Assay, Transduction, Fluorescence, Marker



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    sfg retroviral vector backbone  (TaKaRa)
    99
    TaKaRa sfg retroviral vector backbone
    Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of <t>retroviral</t> vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.
    Sfg Retroviral Vector Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sfg retroviral vector backbone/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    sfg retroviral vector backbone - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier

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    Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of retroviral vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Transgenic CD8αβ co-receptor rescues endogenous TCR function in TCR-transgenic virus-specific T cells

    doi: 10.1136/jitc-2020-001487

    Figure Lengend Snippet: Generation and characterization of TCR+ and TCR8+ VSTs. (A) Schemes of retroviral vectors used in the study. (B) Scheme of transgenic VST production. S1: first stimulation, S2: second stimulation. (C) Transduction efficiencies of VSTs with TCR (red dots) or TCR8 (blue dots) vectors compared with NT controls (gated on live cells, %mTCR+ cells, n=9, mean±SD). (D) CD8α mean fluorescence intensity in VSTs (n=9, mean±SD). (E) Fold expansion of NT (white), TCR+ (red) and TCR8+ (blue) VSTs after S1 and S2 (n=9, mean±SD, p=ns). (F) Distribution of CD4+, CD8+, NK (CD56+ CD3−) or TCRγδ (TCRγδ+ CD3+) cells in NT, TCR+ and TCR8+ VSTs (n=5, mean±SD, p=ns). (G) Memory phenotype: T N , T CM , T EM , and T EFF subset distribution in NT or transduced VSTs based on two-marker CD45RO/CD62L gating (n=5, mean±SD, one-way analysis of variance). CD4+ T N : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: **p=0.008. CD4+ T CM : NT versus TCR8+: *p=0.03; TCR+ versus TCR8+: p=ns. (A to F) Coding of significance levels: *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CMV, cytomegalovirus; DC, dendritic cell; EBV, Epstein-Barr virus; NT, non-transduced; PBMC, peripheral blood mononuclear cell; TCR, T-cell receptor; VSTs, virus-specific T cells.

    Article Snippet: Genes encoding for the human CD8α (Uniprot P01732) and CD8β isoform 1 (βM1, Uniprot P10966-1) chains, separated by a 2A sequence, were synthesized by Geneart (Invitrogen) and cloned into the SFG retroviral vector backbone ( ) (In-Fusion HD Cloning Kit, Clontech).

    Techniques: Transgenic Assay, Transduction, Fluorescence, Marker